Recombinant human trypsin

In the recombinant human trypsin, the sequence and the nature of amino acid is completely same with human trypsin. Trypsin is a kind of endopeptidase, it can be used to shear peptide bond of lysine and C end of arginine, thus split the macromolecular protein into small peptide.

It is gene engineering produced, non animal source, no virus pollution, non hybrid enzyme pollution

Qualification:

Items	Content
Sources	recombinant Escherichia coli
Purification	HPLC
Properties	white or off white freeze-dried powder
Electrophoresis and Coomassie brilliant blue staining 12%SDS-PAGE	Non reduced SDS-PAGE protein electrophoresis, the molecular weight is24kD, a single main band.
Specific activity	≥2500 USPu/mgpro. ≥3800USPu/mgpro
Other enzyme content	not polluted by chymotrypsin and carboxypeptidase A and activity
It does not contain any protease inhibitors	It doesn’t contain any PMSF, EDTA and other protease inhibitors

Activity unit：25℃，pH7.6，reaction system 3.0ml(1cm light path)，each minute emphasis BAEE make absorption value below 253nm increase 0.003 , it is a USP unit.

Packing:10mg,100mg,1g, bulk

Trypsin is widely used in various biotechnological processes, such as:

1) cell separation of various cell cultureed tissue

2) degradation of modified protein

3) protein enzymolysis and sequencing

4) cells treatment of cancer, etc.

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