DNP Antigens and Antibodies - a High-caliber C-line Quality Control System
Time：2023-12-14 Hits：191
Immunochromatographic tests frequently employ anti-mouse or anti-rabbit secondary antibodies, which, due to their C-line nature, frequently introduce heterophilic antibodies (e.g., human anti-animal antibodies, HAAA) into clinical samples. This incorporation can lead to instability in the C-line signal, thus perturbing the accuracy of the test outcomes. Seebio offers DNP antigen and antibody, a robust, interference-free, and superior C-line quality control system for immunochromatography platforms, ensuring reliable results.
For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578
Principle of DNP Antigen and Antibody as an Independent C-line
DNP (Dinitrophenol) is a small molecule with a molecular weight of 184.11, primarily utilized in organic synthesis and as a dye intermediate. Being absent from human metabolism, DNP avoids the generation of non-specific reactions with sample interferences like heterophilic antibodies and human anti-animal antibodies. The cross-linking of DNP with BSA and the production of anti-DNP antibodies creates an ideal candidate for use in immunochromatographic methods, serving as an effective independent quality control system and markedly enhancing C-line specificity.
Product Description
DNP antigen (DNP-BSA）
Item number: EDD0405A
Molecular weight: 66 KD
Appearance: solution
Purity: ≥90% (SDS-PAGE）
Buffer: 50mM Tris-HCl, pH8.0
Application: detection
DNP mouse monoclonal antibody
Item number：EKY0817A
Molecular weight: 150 KD
Appearance: solution
Purity: ≥90% (SDS-PAGE）
Buffer: 10mM PBS, pH7.4
Subtype: IgG1
Application: capture
Recommended usage
In order to better avoid the HAMA effect, it is recommended to coat with DNP antibodies and label with DNP-BSA
Recommended concentration：
Colloidal gold
DNP-BSA label: 20ug/ml；
DNP-BSA label: 20ug/ml；
DNP antibody coating: 1.5mg/ml
Immunofluorescence
DNP-BSA label: 0.4-0.6mg protein: 1ml microspheres；
DNP-BSA label: 0.4-0.6mg protein: 1ml microspheres；
DNP antibody coating:0.5-1mg/ml
Storage conditions
Please store at 2-8°C for short term and -20°C for long term; avoid repeated freezing and thawing.
In addition to using DNP antigen antibodies as independent C lines as a solution to avoid HAMA effects, Seebio can also provide excellent HBR active blockers to significantly reduce the impact of HAMA effects.