Fluorescein isothiocyanate (FITC)

Fluorescein isothiocyanate (FITC), biochemical grade, [3326-32-7], is a green fluorescein derivative widely used in biology, with high absorption rate, good fluorescence properties and water solubility. Its isothiocyanate group can combine with a variety of groups on proteins and is used to label antibodies and lectins. FITC can also be used as a fluorescent tracer for rapid identification of pathogens and in micro-sequencing of proteins and peptides. The excitation wavelength is 494 nm, the emission wavelength is 520 nm, and the fluorescence is yellow-green.

Product properties
Product name: Fluorescein isothiocyanate (FITC)
Alias: Fluorescein isothiocyanate
CAS number: 3326-32-7
Molecular formula:C21H11NO5S
Molecular weight:389.381
H-bond acceptors: 6
H-bond donor: 6
Twist key: 0
Hydrophobic parameter: 4.69
Appearance and Odor: Orange powder
Melting/boiling point: Melting point >360 °C(lit.)
Content/Specification: 95%
Usage: biochemical research, fluorescent antibody tracing
Storage conditions: 2-8℃, protected from light
Hazard description: R22 R36/37/38 R42/43
Security description: S22 S26 S36/37/39
Hazard symbol: Xn


FITC is a green fluorescein derivative widely used in biology, with high absorbance, good fluorescence properties and water solubility. Its isothiocyanate group can combine with a variety of groups on proteins and is used to label antibodies and lectins. FITC can also be used as a fluorescent tracer for rapid identification of pathogens and in micro-sequencing of proteins and peptides. The excitation wavelength is 494 nm, the emission wavelength is 520 nm, and the fluorescence is yellow-green.